Interspecific potato somatic hybrids between Solanum malmeanum and S. tuberosum provide valuable resources for freezing-tolerance breeding

Freezing stress affects the geographic distribution, growth, and development of potato, resulting in yield loss. Solanum malmeanum, a diploid wild species with strong freezing tolerance, was fused sensitive dihaploid S. tuberosum by somatic hybridization. In our study, 980 calli were obtained, 248 differentiated shoots obtained from calli. Parental-specific SSR markers used to analyse chromosome composition 80 randomly selected regenerated plants, obtaining 51 hybrids. Among them, 44 hybrids tested ploidy analysis years 2016 2020. During subculture, genomic levels changed due unstable 56.82% The showed better tolerance than cultivated parent. Then, freezing-tolerant backcross cultivars, we valuable breeding resources enhanced tuberization capacity similar that cultivars. correlation has no relation capacity, which indicates they are controlled independent genetic loci. transferred potato malmeanum protoplast fusion for first time, obtained.